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Blasticidin solution, 10mg/ml in 20mM HEPES

nr kat.: B007-20ML
Opakowanie: 20 mL
Cena brutto: 2 685,09 zł 2685.09
Cena netto: 2 183,00 zł
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Producent: TOKU-E
nr kat.: B007-20ML

Opis

Produkt przeznaczony wyłącznie do badań. Nie nadaje się do spożycia. Więcej informacji na stronie producenta www.toku-e.com


CAS Number: 3513-03-9
Molecular Formula: C17H26N8O5 · HCl
Form: Solution
Source: Streptomyces Griseochromogenes
Storage Conditions: 20°C
 

Description

Blasticidin S HCl is a nucleoside antibiotic derived from Streptomyces griseochromogenes. Blasticidin S HCl is routinely used in cell culture gene selection applications as a selection agent to isolate mammalian cells containing the blasticidin S-resistance (bsr) gene. Blasticidin S HCl solution contains 10 mg/mL blasticidin S HCl in 20mM HEPES. 10 x 1 mL contains 10 mg blasticidin S HCl per vial (100 mg total). 20 mL contains 200 mg blasticidin S HCl.
Blasticidin S HCl in powder form is also available.

Mechanism

Blasticidin S HCl inhibits protein synthesis in prokaryotic and eukaryotic cells by binding to the ribosomal P-site.. Blasticidin S HCl strengthens tRNA binding to the P-site which slows down and prevents subsequent peptide synthesis.

Microbiology Applications

Blasticidin S HCl can be used as a selection agent after transformation of prokaryotic (bacterial) cells.

Mammalian Biology Applications
Blasticidin S HCl is frequently used as a selection agent after transfection of eukaryotic cells. Selectable markers include blasticidin S deaminase resistant genes bsr and bsd.

For more information on relevant cell lines, culture medium, and working concentrations, please visit the TOKU-E Cell-culture Database.

References

Yamaguchi, I., et al. "Expression of the Blasticidin S Deaminase Gene (bsr) in Tobacco: Fungicide Tolerance and a New Selective Marker for Transgenic Plants." Molecular and General Genetics 223.2 (1990): 332-34. www.ncbi.gov. Web. 7 Sept. 2012.

"Blasticidin S Inhibits Translation by Trapping Deformed TRNA on the Ribosome." PNAS 110.30 (2013): 12283-2288. Ncbi.nlm.nih.gov. Web. 4 Sept. 2014.


Dane techniczne

Opakowanie 20 mL

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