Produkty
Producenci

Bacillus megaterium vector pN-His-TEV1622

nr kat.: BMEG22
Opakowanie: 10 µg
Cena brutto: do wyceny
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Opis

All plasmids of the here described series contain the strong xylA promoter originating from Bacillus megaterium. Transcription from this promoter is xylose inducible. After xylose addition, the xylose repressor coded by the xylR gene on the plasmids is released from PxylA and transcription is initiated.

The most convenient cloning sites for insertion of DNA fragments carrying heterologous genes are located in the reading frame of xylA (see sequences). Therefore, any gene can be expressed using one out of three functionally different fusion strategies.

It is important to note that the multiple cloning site (MCS) and its reading frame are identical in all plasmids of this series (without pWH1520) starting from BglII. Hence, a parallel cloning strategy of the gene of interest for tagging with differently located 6xHis, Strep or double-tags or even with the coding sequence of the signal peptide is possible.

For production of target proteins without any tag plasmids for intracellular production (pWH1520, pMM1522, pSTOP1622) and extracellular production (pMM1525) are available, as well as constructs with 6xHis tags (pHIS1522, pC-His1622 & pHIS1525), Strep tags (pSTREP1522, pC-Strep1622 & pSTREP1525), 6xHis/strep double-tags (pSTREPHIS1525), constructs with TEV protease (pN-His-TEV1622, pN-Strep-TEV1622) and Factor Xa protease cleavage site (pN-Strep-Xa1622).

Dane techniczne

Opakowanie 10 µg

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