AlbumiNZ™ Bovine Albumin Low Endotoxin, ≥97%
Opis
- Stem Cell Grade
- From New Zealand-sourced bovine plasma
- Format: Lyophilized powder
- Grade: Cell Culture Grade, ImmunoAssay (EIA) Grade
- Host: Bovine
- Molecular Weight: 66KDa
- pH: 6.5 - 7.5 (4% aq soln)
- Purity: ≥97% Total Protein
Solubility: Albumins are readily soluble in water and can only be precipitated by high concentrations of neutral salts such as ammonium sulfate. The solution stability of albumin is very good (especially if the solutions are stored aliquoted and frozen). Albumin is readily coagulated by heat. When heated to 50 °C or above, albumin quite rapidly forms hydrophobic aggregates which do not revert to monomers upon cooling. Dissolves completely in 20 min at 20-25 °C (20%).
Concentration: Total Protein: ≥95%
Biochemical Physiological Actions
Albumin loses its helical structure rapidly in 2-4 M urea and 4 M guanidinium chloride. The unfolding is reversible, even, to some extent, after concomitant reduction of all disulfide bonds . Reduced albumin can reform into structures capable of binding antibodies and other ligands . Formation of SS bonds is rapid but the return of native structure is slow and some wrong disulfide pairings may persist . After oxidation of the cystine bridges, however, native structure is irreversibly lost.
Application Notes
Albumin binds water, Ca2+, Na+, and K+. Due to a hydrophobic cleft, albumin binds fatty acids, bilirubin, hormones and drugs. The main biological function of albumin is to regulate the colloidal osmotic pressure of blood and to a lesser degree to provide cellular nutrition. Bovine albumin contains 16% nitrogen and is often used as a standard in protein calibration studies. Albumin is used to solubilize lipids, stabilize protein solutions, and is also used as a blocking agent in Western blots or ELISA applications.
Dane techniczne
Opakowanie | 1 kg |