Produkty
Producenci

5-FOA

nr kat.: R0812
Opakowanie: 5 g
Cena brutto: do wyceny
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Opis

The Thermo Scientific 5-Fluoroorotic Acid is a yeast with an active URA3 gene (Ura+) converts 5-FOA to fluorodeoxyuridine, which is toxic to cells (see the scheme below). Yeast strains carrying a mutation in the URA3 gene grow in the presence of 5-FOA if the media is supplemented with uracil. 5-FOA is frequently used in experiments with Saccharomyces cerevisiae (URA3), Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3), and E. coli (pyrF).

Applications

  • Detection of URA3 gene expression in yeast
  • Construction of yeast 2-hybrid libraries
  • Other areas of yeast genetics including gene replacement, analysis of transposable genetic elements, and identification of gene mutations

 Note

  •     Low solubility in water
  •     Solubility in dimethylsulfoxide is greater than 150 mg/mL
  •     Preparation of a 100 mg/mL solution in dime-thylsulfoxide is recommended

Dane techniczne

Opakowanie 5 g
do góry
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