Produkty
Producenci
3,3′,5,5′-TETRAMETHYLBENZIDINE LIQUID SUBSTRATE
Opis
Synonyms: BM blue, Sure Blue TMB, TMB Blotting Plus, TMB substrate, TMB
Concentration: 1.25 mM
Molecular Formula: C16H20N2
Molecular Weight: 240.3
CAS # : 54827-17-7
Form: Stabilized, ready-to-use solution
Storage: Can be stored at room temperature. For long term storage we recommend refrigeration. Let reach room temperature before use. Do not Freeze.
Precaution: Contains dimethylsulfoxide (DMSO), urea peroxide. Avoid contact with the skin or eyes. In case of contact, flush with plenty of tap water. If disposed of in the sink, flush with plenty of tap water to avoid creating a DMSO stench.
Note: If an amorphous precipitate is observed or develops during storage, it can be removed by filtration (filter: Whatmann No. 42 or 44). The amorphous precipitate does not hamper the quality and does not demonstrate instability of the solution.
Do not use if the solution darkens. A slight greenish appearance is acceptable. Very sensitive reagent. Avoid any kind of contamination.
Use in ELISA Reactions -- Basic Reaction Conditions:
| Activating Reagents: | Peroxidase and pseudo-peroxidase activities |
| Sensitivity to day and artificial light: | Negligible |
| Volume per microliter plate well (96-well microtiter plate): | 200 microliters |
| Reaction Time: | 30 minutes |
| Reaction Temperature: | Room Temperature (approx. 25°C) |
| Reaction pH Range: | 4.5 - 5.5 |
| Product Form: | Green to Dark Brown Soluble Chromogen |
| Peak wavelength before stopping: | Two peaks, the major 370-450 nm |
| Stopping Solution: | 2 M Sulfuric Acid (H2SO4) |
| Volume of Stopping Solution per microtiter plate well: | 50 microliters |
| Product form after stopping: | Yellow to Deep Yellow soluble chromogen |
| Peak wavelength after stopping: | One peak 440-450 nm |
| Developed chromogen stability: | 30 minutes |
Solubility: Soluble in water
Dane techniczne
| Opakowanie | 50 mL |