Description: In molecular biology, TBE and TAE buffers are used for agarose and polyacrylamide gel electrophoresis. TBE buffer is suitable when analysing DNA fragments from PCR amplification, DNA isolation protocols, or DNA cloning experiments. It is adapted for separating smaller DNA fragments (less than 1500 bp on a 0.8% agarose gel). TBE has a greater buffering capacity and will give sharper resolution than TAE. However, TBE gels in general afford a poor recovery of nucleic acids compared with TAE gels.
Applications:
Nucleic acid electrophoresis running buffer for agarose and polyacrylamide gels
Directions for use: Dilute 100 mL of TBE Buffer, 10x stock solution into 900 mL deionised water to make 1 litre of TBE Buffer. Final concentration is 89 mM Tris base, 89 mM Borate and 2 mM Na2EDTA. On dilution to 1X the user should check pH and adjust as required.
Specifications:
– Tris 0.9 M 108 g/L
– Boric acid 0.9 M 55 g/L
– Na2EDTA 0.02 M 43.5 g/L
pH 8.3
Storage: Store at room temperature. Keep away from light.
B52
