1-Step™ NBT/BCIP Substrate Solution
Opis
Description
The combination of NBT (nitro-blue tetrazolium chloride) and BCIP (5-bromo-4-chloro-3'-indolyphosphate p-toluidine salt) yields an intense, insoluble black-purple precipitate when reacted with alkaline phosphatase, a popular enzyme conjugate for antibody probes. Pierce NBT and BCIP powders are available individually and in two convenient, ready-to-use solutions (with and without a levamisole suppressor of endogenous phosphatase activity).
Highlights:
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AP substrate – for detection of alkaline phosphatase (alk-phos) activity on solid media, including nitrocellulose and PVDF membranes and fixed tissue samples
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Chromogenic – no special equipment needed for visualization; produces purple-black precipitate that is easy to photograph
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Package options – choose individual NBT and BCIP powders, or preformulated, ready-to-use solutions with or without levamisole suppressor
1-Step™ NBT/BCIP
- Ideal for immunoblotting applications
- Minimal assay-to-assay variability
1-Step NBT/BCIP Plus Suppressor
- Ideal for staining tissues assayed with calf intestinal alkaline phosphatase without the interference caused by endogenous phosphatases
- Contains 1mM levamisole to inhibit endogenous phosphatase activity
Formulation | Proprietary solution of NBT and BCIP |
Quantity | 250mL |
Storage Requirements | 4°C |
Applications | Alkaline phosphate detection |
Detection | Chromogenic |
Kit Contents | Single component |
Sufficient For | 25 uses at 10mL each |
Dane techniczne
Opakowanie | 250 ml |