β-GALACTOSIDASE
Opis
≥300 u/mg solid
Pβ-Galactosidase is useful for the structural investigation of carbohydrate, the determination of lactose (foodstuff analysis) and as an enzyme label for enzyme immunoassay.
Product Description
β-Galactosidase cleaves terminal galactose residues, which are β -1-->14 linked to a monosaccharide, glycopeptide, or oligosaccharide. The enzyme requires Mg2+ for optimal activity.
Biochem/physiol Actions
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.
Unit Definition
One unit catalyzes the hydrolysis of 1 μmole of o-nitrophenyl-β-D-galactopyranoside per minute at 25 °C, pH 7.5.
Inhibitors: p-Chloromercuribenzoate, lodoacetamide, heavy metal ions (Zn++, Fe++, Zn++, Cd++, Cu++, Pb++, Ag+, Hg++), Ionic Detergents (SDS, DAC, etc.). Contaminants: The preparation is practically free from other glycosidases (a-galactosidase, a-,b-glucosidase, a-,b-mannosidase, etc.) and proteinase. Principle: o-Nitrophenyl-b-D-galactopyranoside (ONPG) b-galactosidase > o-Nitrophenol (ONP) + D-Galactose. The appearance of o-nitrophenol is measured at 410 nm by spectrophotemetry. Thermal Stability: Below 50 °C (pH 7.3, 15 min) (Lit.), Optimum Temperature: 50 - 55 °C (Lit.)
Dane techniczne
Opakowanie | 1 KU |