Features
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Fast – complete digestion of genomic DNA in 1 hour at 37°C.
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Efficient – specially formulated enzymes for epigenetic studies of DNA methylation status.
Applications
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CpG methylation analysis at 5’-CCGG-3’ loci.
Description
5-Methylcytosine is a prominent epigenetic DNA modification which plays an important role in regulation of gene expression.
The EpiJET™ DNA Methylation Analysis Kit (MspI/HpaII) uses the MspI and HpaII restriction enzymes to analyze DNA methylation status at a specific locus. Epi MspI and Epi HpaII are isoschizomers with differing sensitivities to CpG methylation. When the internal CpG in the 5’-CCGG-3’ tetranucleotide sequence is methylated, cleavage with Epi HpaII is blocked, but cleavage with Epi MspI is not affected.
The Epi MspI and Epi HpaII enzymes are specially formulated for epigenetics studies to complete genomic DNA digestion in 1 hour.
The EpiJET™ DNA Methylation Analysis Kit (MspI/HpaII) uses the MspI and HpaII restriction enzymes to analyze DNA methylation status at a specific locus. Epi MspI and Epi HpaII are isoschizomers with differing sensitivities to CpG methylation. When the internal CpG in the 5’-CCGG-3’ tetranucleotide sequence is methylated, cleavage with Epi HpaII is blocked, but cleavage with Epi MspI is not affected.
The Epi MspI and Epi HpaII enzymes are specially formulated for epigenetics studies to complete genomic DNA digestion in 1 hour.
Quality Control
Functional Testing
The kit is functionally tested in Epi MspI and Epi HpaII digestion of human blood genomic DNA, spiked with methylated and unmethylated pUC19DNA/SmaI. Unmethylated plasmid and genomic DNA are completely cleaved in one hour with both enzymes. Methylated plasmid and genomic DNA are not cleaved by Epi HpaII, but cleaved by Epi MspI.
The kit is functionally tested in Epi MspI and Epi HpaII digestion of human blood genomic DNA, spiked with methylated and unmethylated pUC19DNA/SmaI. Unmethylated plasmid and genomic DNA are completely cleaved in one hour with both enzymes. Methylated plasmid and genomic DNA are not cleaved by Epi HpaII, but cleaved by Epi MspI.
Components
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Component
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K1441 (200 react.)
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Epi Mspl
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200 µl
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Epi HpaII
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200 µl
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10X EpiBuffer
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1 ml
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Control pUC19/SmaI DNA Unmethylated (0.5 µg/µl)
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20 µl
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Control pUC19/SmaI DNA CpG Methylated (0.5 µg/µl)
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20 µl
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Order Information
K1441 - 200 reactions
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