For FRET Interaction Studies
Nuclear receptor fusion proteins are well suited for protein-cofactor peptide interaction studies. NR Ligand Binding Domains, as one binding partner, can be used to test for biotinylated peptides with sequences around the cofactors’ LXXLL motifs as the other binding partner.
Principle of FRET Assay System: The FRET assay principle is the energy transfer, when Eu-chelate as fluorescent donor and allophycocyanin as fluorescent acceptor come close to each other. If the titrated ligand modulates the interaction between Nuclear Receptor and peptide, the fluorescent signal is modulated in a ligand dose-dependent manner.
Features
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Recombinant, affinity-purified NR LBD fusion proteins
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Useful for various assays of disease relevant NR targets
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Functionally validated to ensure high-quality results each and every time
Order Information
Please click on the respective order # for datasheets.
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order #
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description
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amount
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Nuclear Receptor Fusion Proteins
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Nuclear receptor PPAR alpha NR1C1 LBD (aa164-468)
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100 FRET assay points
(80µl, ~100µg estimated from SDS-PAGE) |
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Nuclear receptor PPAR beta/delta NR1C2 LBD (aa137-441)
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100 FRET assay points
(50µl, ~20µg estimated from SDS-PAGE) |
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Nuclear receptor PPAR gamma-fl NR1C3 LBD (aa1-477)
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100 FRET assay points
(50µl, ~5µg estimated from SDS-PAGE) |
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Nuclear receptor LXR beta LBD (aa155-461)
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100 FRET assay points
(50µl, ~10µg estimated from SDS-PAGE) |
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Nuclear receptor FXR (mouse) NR1H4 LBD (aa202-484)
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100 FRET assay points
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Nuclear receptor VDR NR1I1 LBD (aa88-428)
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100 FRET assay points
(50µl, ~10µg estimated from SDS-PAGE) |
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Nuclear receptor PXR (SXR) NR1I2 LBD (aa106-437)
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100 FRET assay points
(50µl, ~15µg estimated from SDS-PAGE) |
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Nuclear receptor ERR alpha NR3B1 LBD (aa143-423)
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100 FRET assay points
(50 µl, ~5 µg estimated from SDS-PAGE) |
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Nuclear receptor ERR gamma NR3B3 LBD (aa190-423)
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100 FRET assay points
(100 µl, ~10 µg estimated from SDS-PAGE) |
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