The cDNA libraries listed below can be used in combination with the Y2H Membrane Protein Kit or the Y2H Transactivating Protein System to identify interaction partners.
Order information, shipping, storage:
|
order#
|
description
|
amount
|
|
Saccharomyces cerevisiae cDNA library (NubG-x)
|
200 µg NEW!
|
|
|
Normalized mouse embryo cDNA library (x-NubG)
|
200 µg NEW!
|
|
|
Jurkat T cDNA library (NubG-x)
|
200 µg
|
|
|
Mouse adult heart cDNA library (x-NubG)
|
200 µg
|
|
|
Mouse adult heart cDNA library (NubG-x)
|
200 µg
|
|
|
Human adult kidney cDNA library (x-NubG)
|
200 µg
|
|
|
Arabidopsis cDNA library (NubG-x)
|
200 µg
|
|
|
HeLa cell line cDNA library (NubG-x)
|
200 µg
|
|
|
Human embryonal brain cDNA library (NubG-x)
|
200 µg
|
|
|
Human adult colon cDNA library (NubG-x)
|
200 µg
|
|
|
C. elegans whole adult cDNA library (NubG-x)
|
200 µg
|
|
|
Human adult liver cDNA library (NubG-x)
|
200 µg
|
|
|
Human adult brain cDNA library (NubG-x)
|
200 µg
|
|
|
C. elegans whole eggs DNA library (NubG-x)
|
200 µg
|
|
|
D. melanogaster embryo cDNA library (NubG-x)
|
200 µg
|
|
|
Mouse adult spleen cDNA library (x-NubG)
|
200 µg
|
|
|
Human adult kidney cDNA library (NubG-x)
|
200 µg
|
|
|
Human adult brain cDNA library (x-NubG)
|
200 µg
|
|
|
LNCaP cells cDNA library (NubG-x)
|
200 µg
|
|
|
Drosophila melanogaster cDNA library (NubG-x)
|
200 µg
|
|
|
Human adult lung cDNA library (NubG-x)
|
200 µg
|
|
|
Mouse adult kidney cDNA library (NubG-x)
|
200 µg
|
|
|
Mammary epithelial cells cDNA library (NubG-x)
|
200 µg
|
|
|
Rat neonatal cardiomyocyte cDNA library (NubG-x)
|
200 µg
|
|
|
Mouse whole embryo, 11 days cDNA library (NubG-x)
|
200 µg
|
|
|
Medicago nodules cDNA library (x-NubG)
|
200 µg
|
|
|
Medicago nodules cDNA library (NubG-x)
|
200 µg
|
|
|
shipped at RT; store plasmid DNA at -20°C
|
||
All libraries have average insert sizes > 1.5 kb and complexities > 2x106 independent clones. Libraries are supplied as 200 µg purified plasmid DNA, ready for yeast transformation. This amount is sufficient for 5 screens.
The cDNA libraries for the Y2H Membrane Protein System may be constructed in two orientations: NubG-x
and x-NubG
.
NubG-x
libraries express fusions of NubG to the N-terminus of the prey. They are suitable for identifying cytosolic proteins or Type II integral membrane proteins as binding partners.
x-NubG
libraries express fusions of NubG to the C-terminus of the prey. They are suitable for identifying cytosolic proteins or Type I integral membrane proteins as binding partners.
Other cDNA Libraries for Conventional Yeast Two-Hybrid Screens
The cDNA libraries listed below express cDNAs as fusions to the GAL4 activation domain. They can be used with most commercially available yeast two-hybrid systems (see Conventional Yeast Two-Hybrid Vectors). Libraries have been carefully constructed from polyA+-RNA and rigorously tested with regard to quality. The prey vector used for library construction is pGAD-DS .
Libraries are delivered as 2 x 100 µg purified plasmid DNA, ready for yeast transformation. This amount is sufficient for 7 screens.
Order information, shipping, storage:
|
order#
|
description
|
amount
|
|
P02101DS
|
Mouse 11d total embryo / pGAD-DS
|
200 µg
|
|
P02102DS
|
Human colon / pGAD-DS
|
200 µg
|
|
P02103DS
|
HeLa cell line
|
200 µg
|
|
shipped at RT; store at -20°C
|
||
Eukaryotic LambdaPS Libraries
Features and Advantages:
-
large insert size (up to 20 kb)
-
convenience of working with high copy-plasmids
-
complete representation of the genome (at least 5-fold)
-
automatic subcloning facility
-
competitive price (especially for kits of several libraries!)
-
of special interest: the pufferfish model vertebrate genome
Applications:
-
screening
-
easy characterization of clones
-
homology studies
-
pufferfish as model for discovery of human genes
-
gene targeting (mouse library: strain 129, congenic with popular ES cell lines)
-
developmental, evolutionary and genetic studies on vertebrates and non-vertebrates
Characterization of LambdaPS Libraries:
|
Species
|
Average
insert size |
Redundancy
(about) |
Suggested # of
phage per plating |
|
human (male; peripheral blood leukocytes)
|
17.5 kb
|
20
|
2 x 10 6
|
|
mouse (129/Sv, male, embryonal stem cell line)
|
17 kb
|
4
|
2 x 10 6
|
|
rat (Sprague-Dawley)
|
18 kb
|
10
|
2 x 10 6
|
|
zebrafish (Danio rerio)
|
17 kb
|
5
|
2 x 10 6
|
|
pufferfish (Fugu rubripes)
|
17 kb
|
8
|
2 x 10 5
|
|
D. melanogaste
r (Canton S)
|
20 kb
|
500
|
2 x 10 5
|
|
C. elegans
|
17 kb
|
50
|
2 x 10 5
|
|
S. cerevisiae
(YNN295)
|
17.5 kb
|
10
|
2 x 10 4
|
Product Description:
In comparison to other library systems, genomic libraries created in the vector lambdaPS facilitate screening and subsequent characterization of clones significantly. Since the replacement vector of phage origin can accommodate up to 20 kb DNA inserts, complex genomes are completely represented in a reasonable number of recombinants (approx. 5-fold coverage). For characterization of selected clones the elegant system features an automatic plasmid subcloning facility of insert fragments: linear lambdaPS phage contains two loxP sites in direct orientation flanking a high copy plasmid backbone and the insert. Recombination between these two sites is mediated by Cre recombinase (in the re-expressing E. coli strain BNN132). This leads to excision of the multi-copy plasmid from the phage genome obviating the need for subcloning the insert into another system. Thus, time-consuming purification of large amounts of phage DNA for characterization is now superfluous. A restriction map is easily compiled using the excised plasmid, which can be further manipulated.
Of special interest to the researcher should be the library from the pufferfish genome (Fugu rubripes), which is 7.5 times smaller than the human genome. Because of its similar gene repertoire it is the best model for the discovery of human genes. The lambdaPS phage for construction of custom made libraries is also available for the experienced researcher.
Of special interest to the researcher should be the library from the pufferfish genome (Fugu rubripes), which is 7.5 times smaller than the human genome. Because of its similar gene repertoire it is the best model for the discovery of human genes. The lambdaPS phage for construction of custom made libraries is also available for the experienced researcher.
Automatic subcloning: Site-specific recombination between loxP sites catalysed by Cre-recombinase from E. coli BNN132
Order information, shipping, storage:
|
order#
|
description
|
amount
|
|
1 ml phage lysate each; host strains included:
|
||
|
PS01
|
1. human genomic lambdaPS library
|
1 ml
|
|
PS02
|
2. mouse genomic lambdaPS library
|
1 ml
|
|
PS03
|
3. rat genomic lambdaPS library
|
1 ml
|
|
PS04
|
4. zebrafish genomic lambdaPS library
|
1 ml
|
|
PS05
|
5. pufferfish genomic lambda PS library
|
1 ml
|
|
PS06
|
6. C. elegans genomic lambdaPS library
|
1 ml
|
|
PS07
|
7. D. melanogaster genomic lambdaPS library
|
1 ml
|
|
PS08
|
8. yeast genomic lambdaPS library
|
1 ml
|
|
PS15
|
Vertebrate kit (#1-5)
|
1 ml each
|
|
PS68
|
Non-vertebrate kit (#6-8)
|
1 ml each
|
|
PS18
|
Complete kit (#1-8)
|
1 ml each
|
|
PS10
|
lambdaPS phage lysate
|
1 ml
|
|
shipped on dry ice; store at -70°C
|
||
Download: Handbook
Logowanie
Centrum zamówień
Kontakt