The Lift-Off Detaching Reagent contains protease, and collagenase activities in an isotonic phosphate buffer solution with EDTA. Lift-Off detaching reagent provides quick and gentle detachment and dissociation of a wide variety of adherent cells, including primary cells, grown in all known tissue culture plastic ware. The Lift-Off Detaching Reagent is suitable for detaching adherent cells from in vitro growth vesicles. Lift-Off detaching reagent offers the following benefits: