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Mobitec
The new innovative system for easy labeling of DNA and RNA! Click-chemistry
Click-Chemistry
Features
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Fast and purposeful chemical reaction with high yields.
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Convenient labeling of RNA or DNA via solid-phase synthesis (Phosphoramidites) or via PCR with high efficiency.
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Up to three different modifications onto one position of the same DNA strand.
Description
Click-chemistry was first introduced by K. Berry Sharpless in 2001. It describes a chemical reaction which builds substances by joining small units together. Click-reactions are fast and purposeful with high yields.
The most popular click-reaction is azide alkyne Huisgen cycloaddition with Copper (Cu) as catalyst at room temperature.
Now you can use this highly efficient technology for labeling of DNA or RNA via solid-phase synthesis or PCR. Furthermore, it is possible to introduce up to three different modifications onto one position of the same DNA strand. Of course, we offer different kinds of non-fluorescent and fluorescent labels.
The most popular click-reaction is azide alkyne Huisgen cycloaddition with Copper (Cu) as catalyst at room temperature.
Now you can use this highly efficient technology for labeling of DNA or RNA via solid-phase synthesis or PCR. Furthermore, it is possible to introduce up to three different modifications onto one position of the same DNA strand. Of course, we offer different kinds of non-fluorescent and fluorescent labels.
For labeling via solid-phase synthesis (Fig. 1) MoBiTec offers Phosphoramidites which enable the synthesis of oligonucleotides with reactive triple bonds for the attachment of different labels.
Another possibility is the generation of DNA strands with reactive triple bonds by PCR through integration of our C8-Alkyne triphosphates.
Another possibility is the generation of DNA strands with reactive triple bonds by PCR through integration of our C8-Alkyne triphosphates.
Fig. 1 Schematic overview of solid phase synthesis
For labeling you can use either non- fluorescent labels like Biotin- or Dabcylazides or fluorescent azides/NHS. MoBiTec offers a great range of different fluorescent dyes for click-chemistry. In addition to Fluorescein-Azide we supply various MFP-Eterneon-Azides/-NHS. Our MFP-Eterneon-Dyes have a low molecular weight and good solubility. Most notably, with their great Stokes shift they are convenient for cell cytometry.
Ancillary material like CuBr or Click-Solution is available as well.
Fig. 2 DNA can be modified postsynthetically using click chemistry. The attachment of up to three different labels is possible on any position of the DNA strands, multiple functionalizations with the same labeling moiety is possible as well.
Order information, Shipping & Storage
Phosphoramidites
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Order Information
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Order #
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Description
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Amount
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C8-Alkyne-dC-CEP
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250 mg
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C8-Alkyne-dU-CEP
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250 mg
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C8-TIPS-dC-CEP
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250 mg
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Triphosphates
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Order Information
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Order #
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Description
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Amount
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C8-Alkyne-dUTP
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0,5 µmol
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C8-Alkyne-dUTP
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2,5 µmol
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C8-Alkyne-dUTP
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5,0 µmol
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C8-Alkyne-dCTP
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0,5 µmol
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C8-Alkyne-dCTP
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2,5 µmol
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C8-Alkyne-dCTP
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5,0 µmol
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Azides
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Order Information
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Order #
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Description
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Amount
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Dabcyl-Azide
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1 mg
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Biotin-Azide
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1 mg
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Dabcyl-Azide
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5 mg
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Biotin-Azide
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5 mg
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Dabcyl-Azide
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100 mg
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Biotin-Azide
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100 mg
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Fluorescence Dyes
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Order Information
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Order #
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Description
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Amount
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Fluorescein-Azide (6-FAM)
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1 mg
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Fluorescein-Azide (6-FAM)
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5 mg
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Fluorescein-Azide (6-FAM)
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100 mg
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MFP-Eterneon-350/430 Azide
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1 mg
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MFP-Eterneon-350/455 Azide
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1 mg
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MFP-Eterneon-384/480 Azide
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1 mg
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MFP-Eterneon-393/523 Azide
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1 mg
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MFP-Eterneon-394/507 Azide
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1 mg
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MFP-Eterneon-480/635 Azide
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1 mg
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MFP-Eterneon-350/430 NHS
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1 mg
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MFP-Eterneon-350/455 NHS
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1 mg
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MFP-Eterneon-384/480 NHS
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1 mg
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MFP-Eterneon-393/523 NHS
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1 mg
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MFP-Eterneon-394/507 NHS
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1 mg
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MFP-Eterneon-480/635 NHS
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1 mg
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MFP-Eterneon-350/430 Azide
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5 mg
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MFP-Eterneon-350/455 Azide
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5 mg
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MFP-Eterneon-384/480 Azide
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5 mg
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MFP-Eterneon-393/523 Azide
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5 mg
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MFP-Eterneon-394/507 Azide
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5 mg
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MFP-Eterneon-480/635 Azide
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5 mg
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MFP-Eterneon-350/430 NHS
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5 mg
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MFP-Eterneon-350/455 NHS
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5 mg
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MFP-Eterneon-384/480 NHS
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5 mg
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MFP-Eterneon-393/523 NHS
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5 mg
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MFP-Eterneon-394/507 NHS
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5 mg
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MFP-Eterneon-480/635 NHS
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5 mg
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MFP-Eterneon-350/430 Azide
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10 mg
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MFP-Eterneon-350/455 Azide
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10 mg
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MFP-Eterneon-384/480 Azide
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10 mg
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MFP-Eterneon-393/523 Azide
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10 mg
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MFP-Eterneon-394/507 Azide
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10 mg
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MFP-Eterneon-480/635 Azide
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10 mg
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MFP-Eterneon-350/430 NHS
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10 mg
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MFP-Eterneon-350/455 NHS
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10 mg
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MFP-Eterneon-384/480 NHS
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10 mg
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MFP-Eterneon-393/523 NHS
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10 mg
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MFP-Eterneon-394/507 NHS
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10 mg
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MFP-Eterneon-480/635 NHS
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10 mg
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Miscellaneous
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Order Information
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Order #
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Description
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Amount
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CuBr (click grade)
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5 mg
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CuBr (click grade)
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10 x 5 mg
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TBTA-Ligand (click grade)
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5 mg
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TBTA-Ligand (click grade)
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10 mg
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Click-Solution (DMSO/t-Butanol)
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1.0 ml
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Click-Solution (DMSO/t-Butanol)
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10 x 1.0 ml
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Order Information
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Order #
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Description
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Amount
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NHS-PEG4-Azide
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5 mg
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NHS-PEG4-Azide
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10 mg
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PEG24-Azide
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5 mg
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PEG24-Azide
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10 mg
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PEG8-Azide
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5 mg
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PEG8-Azide
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10 mg
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HO-PEG2-Azide
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5 mg
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HO-PEG2-Azide
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10 mg
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H2N-PEG8-Azide
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5 mg
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H2N-PEG8-Azide
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10 mg
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PEG7-Bis-Azide
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5 mg
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PEG7-Bis-Azide
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10 mg
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4-Azidoaniline Hydrochloride
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5 mg
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4-Azidoaniline Hydrochloride
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10 mg
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4-Azidobenzoic acid
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5 mg
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4-Azidobenzoic acid
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10 mg
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5-Azidopentanoic acid
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5 mg
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5-Azidopentanoic acid
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10 mg
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