MoBiTec TRIO-Extraction Reagent includes phenol and guanidinium thiocyanate in a monophasic solution. After addition of chloroform, the homogenate separates into three phases upon centrifugation. The aqueous phase, interphase and organic phase containing the RNA, DNA and the protein, respectively, are processed separately resulting in RNA, DNA and protein of excellent quality in high yield. This method has been shown to yield undegraded RNA from small and large samples and allows to process a large number of samples simultaneously. Simultaneous purification of DNA is an effective way to normalize RNA yields from sample to sample.
MoBiTec TRIO-Extraction Reagent is stable for 12 months from the date of purchase when stored at 4°C.
Ready-to-use red solution*.
One phase system.
The red color allows easy visualization of the interphase .
1 hour procedure (no CsCl gradient necessary).
Specially designed for liquid samples.
High and reproducible yields.
* Solutions contain phenol (toxic) and guanidinium salts (irritant). Handle with gloves. Avoid contact with eyes, skin or clothes. Avoid breathing vapor.
